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Free-standing lipid vesicles, formed by spherical assemblies of lipid bilayers that enclose an aqueous compartment, are commonly used model membranes, and they are classified by size: small unilamellar vesicles (SUVs), which are less than 50 nm in size, large unilamellar vesicles (LUVs), which are between 100 nm and 1 µm in size, and giant unilamellar vesicles (GUVs), which are larger than 1 µm (Figure 2A)

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